Preparation Saline-sodium citrate (SSC) Buffer
This guide describes the preparation of Saline-sodium citrate (SSC) Buffer at a defined molarity for laboratory use.
SSC buffer is commonly used in nucleic acid hybridization washes.
Saline-sodium citrate (SSC) – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Sodium chloride | NaCl | 0.15 M | 7647-14-5 |
| Sodium citrate | C₆H₅O₇Na₃ | 0.015 M | 68-04-2 |
Sterilization & Storage
- Sterilization: Autoclave
- Storage: Room temperature
Tips
- 2X SSC buffer can be used for high-salt membrane washing to remove partially non-specific probe binding.
- 0.5X SSC buffer can be used for low-salt membrane washing to increase stringency, thereby enhancing the repulsive forces between RNA and DNA strands.
Preview
