Preparation SSPE Buffer
This guide describes the preparation of SSPE Buffer at a defined molarity for laboratory use.
SSPE buffer is commonly used in nucleic acid hybridization experiments.
SSPE Buffer – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Sodium chloride | NaCl | 150 mM | 7647-14-5 |
| Sodium dihydrogen phosphate | NaH₂PO₄ | 10 mM | 64-19-7 |
| EDTA | C₁₀H₁₆N₂O₈ | 1 mM | 60-00-4 |
Sterilization & Storage
- Sterilization: Autoclave
- Storage: Room temperature
Tips
- EDTA (free acid) is extremely difficult to dissolve. It is recommended to prepare an EDTA stock solution first.
- The use of EDTA solution or EDTA·2Na·2H₂O is recommended.
- Anhydrous sodium dihydrogen phosphate is highly hygroscopic; avoid prolonged exposure during weighing.
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