Preparation Tris-Buffered Saline (TBS)
This guide describes the preparation of Tris-Buffered Saline (TBS) at a defined molarity for laboratory use.
TBS buffer is commonly used in molecular biology experiments as a general-purpose buffer.
Tris-Buffered Saline (TBS) – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Tris base | C₄H₁₁NO₃ | 20 mM | 77-86-1 |
| Sodium chloride | NaCl | 150 mM | 7647-14-5 |
Sterilization & Storage
- Sterilization: Autoclave (optional)
- Storage: Room temperature
Tips
- White flocculent precipitates may form during low-temperature storage. Warm in a water bath until fully dissolved before use.
- At a 1X concentration, the addition of 0.05% (v/v) Tween 20 yields TBST Buffer.
- For long-term storage, keep at 4°C.
Preview
