Preparation TNE/STE Buffer
This guide describes the preparation of TNE/STE Buffer at a defined molarity for laboratory use.
TNE buffer is commonly used in molecular biology for nucleic acid and protein applications.
TNE – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Tris-Cl (pH 8.0) | C₄H₁₁NO₃ | 10 mM | 77-86-1 |
| EDTA | C₁₀H₁₆N₂O₈ | 1 mM | 60-00-4 |
| Sodium chloride | NaCl | 100 mM | 7647-14-5 |
Sterilization & Storage
- Sterilization: Autoclave
- Storage: Room temperature
Tips
- At a 1X concentration, the addition of 5% (v/v) Triton X-100 yields STET buffer.
- EDTA (free acid) is extremely difficult to dissolve. It is recommended to prepare an EDTA stock solution first.
- The use of EDTA solution or EDTA·2Na·2H₂O is recommended.
Preview
